全文获取类型
收费全文 | 3446篇 |
免费 | 249篇 |
国内免费 | 318篇 |
出版年
2023年 | 36篇 |
2022年 | 60篇 |
2021年 | 82篇 |
2020年 | 67篇 |
2019年 | 77篇 |
2018年 | 97篇 |
2017年 | 99篇 |
2016年 | 109篇 |
2015年 | 99篇 |
2014年 | 135篇 |
2013年 | 210篇 |
2012年 | 134篇 |
2011年 | 171篇 |
2010年 | 129篇 |
2009年 | 186篇 |
2008年 | 180篇 |
2007年 | 197篇 |
2006年 | 202篇 |
2005年 | 175篇 |
2004年 | 119篇 |
2003年 | 118篇 |
2002年 | 99篇 |
2001年 | 106篇 |
2000年 | 95篇 |
1999年 | 83篇 |
1998年 | 76篇 |
1997年 | 71篇 |
1996年 | 69篇 |
1995年 | 65篇 |
1994年 | 66篇 |
1993年 | 81篇 |
1992年 | 60篇 |
1991年 | 41篇 |
1990年 | 32篇 |
1989年 | 37篇 |
1988年 | 31篇 |
1987年 | 26篇 |
1986年 | 26篇 |
1985年 | 37篇 |
1984年 | 40篇 |
1983年 | 24篇 |
1982年 | 34篇 |
1981年 | 28篇 |
1980年 | 31篇 |
1979年 | 20篇 |
1978年 | 13篇 |
1977年 | 11篇 |
1976年 | 12篇 |
1974年 | 4篇 |
1972年 | 4篇 |
排序方式: 共有4013条查询结果,搜索用时 31 毫秒
31.
Abstract The aim of this study was to use the FAO-56 single and dual crop coefficient approaches to estimate actual evapotranspiration (ETa) over an irrigated citrus orchard under drip and flood irrigations in Marrakech, Morocco. The results showed that, by using crop coefficients suggested in the FAO-56 paper, the performance of both approaches was poor for two irrigation treatments. The Root Mean Squared Error (RMSE) between measured and simulated ETa values over the citrus orchard under drip irrigation was about 1.43 and 1.27 mm/day for the single and dual approaches, respectively, while the corresponding statistics for the orchard irrigated by the flooding technique was 1.87 and 2.48 mm/day. After determination of the appropriate values of the crop coefficient (Kc) based on eddy covariance measurements of ETa, the performance of both approaches greatly improved. The obtained Kc values were lower than the FAO-56 values by about 20%. The low Kc values obtained reflect the practice of drip irrigation for one field and the low value of cover fraction for the other field. Additionally, the efficiency of the irrigation practices was investigated by comparing the measured Kc for two fields. The results showed that a considerable amount of water was lost by direct soil evaporation from the citrus orchard irrigated by flooding technique. 相似文献
32.
Jean‐Baptiste Coutelis Nicanor González‐Morales Charles Géminard Stéphane Noselli 《EMBO reports》2014,15(9):926-937
Differentiating left and right hand sides during embryogenesis represents a major event in body patterning. Left–Right (L/R) asymmetry in bilateria is essential for handed positioning, morphogenesis and ultimately the function of organs (including the brain), with defective L/R asymmetry leading to severe pathologies in human. How and when symmetry is initially broken during embryogenesis remains debated and is a major focus in the field. Work done over the past 20 years, in both vertebrate and invertebrate models, has revealed a number of distinct pathways and mechanisms important for establishing L/R asymmetry and for spreading it to tissues and organs. In this review, we summarize our current knowledge and discuss the diversity of L/R patterning from cells to organs during evolution. 相似文献
33.
34.
Using molecular dynamics (MD) simulations, the density of single proteins and its temperature dependence was modelled starting from the experimentally determined protein structure and a generic, transferable force field, without the need of prior parameterization. Although all proteins consist of the same 20 amino acids, their density in aqueous solution varies up to 10% and the thermal expansion coefficient up to twofold. To model the protein density, systematic MD simulations were carried out for 10 proteins with a broad range of densities (1.32–1.43?g/cm3) and molecular weights (7–97?kDa). The simulated densities deviated by less than 1.4% from their experimental values that were available for four proteins. Further analyses of protein density showed that it can be essentially described as a consequence of amino acid composition. For five proteins, the density was simulated at different temperatures. The simulated thermal expansion coefficients ranged between 4.3 and 7.1?×?10?4?K?1 and were similar to the experimentally determined values of ribonuclease-A and lysozyme (deviations of 2.4 and 14.6%, respectively). Further analyses indicated that the thermal expansion coefficient is linked to the temperature dependence of atomic fluctuations: proteins with a high thermal expansion coefficient show a low increase in flexibility at increasing temperature. A low increase in atomic fluctuations with temperature has been previously described as a possible mechanism of thermostability. Thus, a high thermal expansion coefficient might contribute to protein thermostability. 相似文献
35.
The glutathione content of human erythrocytes rapidly diminishes when cells are exposed to 2,4,6-trinitrobenzenesulfonate (20 μmol/l cells) at 37°C. Even at 0°C a slow decrease in glutathione content is observed. The uptake of trinitrobenzenesulfonate by the cells is retarded by inhibitors of the inorganic anion exchange system, indicating that trinitrobenzenesulfonate enters the cells by this pathway.The disappearance of glutathione most probably results from the reaction: 2 GSH + trinitrobenzenesulfonate → GSSG + aminodinitrobenzenesulfonate The reaction of trinitrobenzenesulfonate with glutathione occurs prior to its covalent binding to amino groups of hemoglobin which makes this reaction a more sensitive method of detection of penetration of trinitrobenzenesulfonate into erythrocytes. Results of studies on the asymmetric distribution of phospholipids using trinitrobenzenesulfonate as the only probe should be reconsidered in the light of these new data. 相似文献
36.
By making use of the capacity of phospholipase A2 to degrade selectively the phospholipid in the outer half of the lipid bilayer of small unilamellar phospholipid/cholesterol vesicles without affecting the retention of a vesicle-encapsulated solute, we demonstrated that the exchange of phosphatidylcholine between such vesicles and human high density lipoprotein involves exclusively the phosphatidylcholine present in the outer monolayer of the vesicle membrane. 相似文献
37.
Petr Bureš František Zedek 《Evolution; international journal of organic evolution》2014,68(8):2412-2420
Similar to how the model of centromere drive explains the size and complexity of centromeres in monocentrics (organisms with localized centromeres), our model of holokinetic drive is consistent with the divergent evolution of chromosomal size and number in holocentrics (organisms with nonlocalized centromeres) exhibiting holokinetic meiosis (holokinetics). Holokinetic drive is proposed to facilitate chromosomal fission and/or repetitive DNA removal (or any segmental deletion) when smaller homologous chromosomes are preferentially inherited or chromosomal fusion and/or repetitive DNA proliferation (or any segmental duplication) when larger homologs are preferred. The hypothesis of holokinetic drive is supported primarily by the negative correlation between chromosome number and genome size that is documented in holokinetic lineages. The supporting value of two older cross‐experiments on holokinetic structural heterozygotes (the rush Luzula elegans and butterflies of the genus Antheraea) that indicate the presence of size‐preferential homolog transmission via female meiosis for holokinetic drive is discussed, along with the further potential consequences of holokinetic drive in comparison with centromere drive. 相似文献
38.
The estimation of soil moisture by using the backscattering coefficient of radar in a mountainous region is a challenging task due to the complex topography, which impacts the distribution of soil moisture and changes the backscattering coefficient. Complicated terrain can disturb empirical moisture estimation models, thereby, the resulting estimates of soil moisture are very unlikely reliable. This article proposed an innovative way of integration of the topographic wetness index (TWI) and the backscattering coefficient of soil obtained from the TerraSAR-X image, which improves the accuracy of measurement of the soil moisture. The standard estimation error and the coefficient of determination from the model were used to evaluate the performance of TWI. Our results show that the standard estimation error was decreased from: (1) 4.0% to 3.3% cm3 cm−3 at a depth of 5 cm and (2) 4.5% to 3.9% cm3 cm−3 at a depth of 10 cm. The most reliable estimation was observed at a depth of 5 cm, when it was compared with those of 0–5 cm, 10 cm and 15 cm. The TWI from the digital elevation model (DEM) is useful as a constraint condition for modeling work. This article concludes that the integration of the backscattering coefficient of soil with TWI can significantly reduce the uncertainty in the estimation of soil moisture in a mountainous region. 相似文献
39.
Draud Matthew; Macias-Ordonez Rogelio; Verga Jack; Itzkowitz Murray 《Behavioral ecology》2004,15(1):102-108
Selection usually acts differently on males and females duringintrasexual competition for resources and/or mates. Nevertheless,agonistic behavior has been examined both theoretically andempirically mostly in males. Our research questions whethermales and females follow the same rules of engagement in intrasexualcontests as predicted by the sequential assessment model (SAM).The SAM predicts negative correlations between contest intensityand duration and the magnitude of asymmetry in resource holdingpower (RHP) between the contestants, such that the most escalatedcontests are those between similarly endowed individuals. Westaged male and female intrasexual contests with varying degreesof body size asymmetry under a round robin design using themonogamous Texas cichlid fish (Herichthys cyanoguttatum) asa study case. We used Mantel's matrix analysis to compare howthe behavioral content, duration, structure, and outcome ofmale and female contests were affected by the relative bodysize of the contestants. In the case of males, relative sizein each contest predicted outcome, duration, and frequency ofconventional and escalated behaviors according to prevailingtheory. Female contest structure and outcome, however, werenot predicted by the relative size of contestants. We discussour results in terms of other asymmetries that might be importantin structuring female contests, and we propose potential approachesto study femalefemale aggression. 相似文献
40.
Growing evidence demonstrates that fluctuating metal stressors can have profound impact on the ecophysiological responses in aquatic species. However, how environmental stochasticity affects the complex damage-recovery dynamics in organisms remains difficult to predict. The objective of this paper was to investigate the stochastic behavior in the damage-recovery dynamics in tilapia in response to pulse waterborne copper (Cu). We developed a mathematical framework that allows discrimination between damage and recovery processes in tilapia exposed to designed pulse Cu scenarios. We built deterministic nonlinear models for the damage-recovery dynamics that produce response surfaces describing killing/recovery rate–Cu-pulse interval interactions. Here we showed that the stochastic switching behavior arose from competition among killing, recovery rates, and Cu pulse frequency. This competition resulted in an ultrasensitivity appeared in whole body, gills, muscle, liver, and kidney with Hill coefficients of ≥7, 4, 7, 5, and 5, respectively, at Cu 3 mg L−1, dilution rate 0.05 h−1, and pulse interval 72 h, indicating that a stochastic switch-like response was generated. We argue that the role of gill-associated Hill coefficient as a direct signal of the stochastic switch-like response in the damage-recovery dynamics in response to pulse metal stressor can serve as a sensitive indicator for risk detection in fluctuating environments. Our approach constitutes a general method to identify the stochastic switch-like response for aquatic species exposed to fluctuating metal stressors, which may help to predict and, eventually, expand our understanding of the damage-recovery dynamics. Finally, we implicate that Hill coefficient-based switch-like signal and its damage with hazard response can be linked in an information theoretic framework to handle environmental stochasticity. 相似文献